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Literature summary extracted from

  • Beassoni, P.R.; Berti, F.P.; Otero, L.H.; Risso, V.A.; Ferreyra, R.G.; Lisa, A.T.; Domenech, C.E.; Ermacora, M.R.
    Preparation and biophysical characterization of recombinant Pseudomonas aeruginosa phosphorylcholine phosphatase (2010), Protein Expr. Purif., 71, 153-159.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.75 expressed in Escherichia coli BL21(DE3) Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.75 hanging drop method Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.75 C87A involved in a non-essential intramolecular disulfide bond Pseudomonas aeruginosa
3.1.3.75 C94A involved in a non-essential intramolecular disulfide bond Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.75 2.9
-
 p-nitrophenyl phosphate
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.75 Mg2+
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.75 37089
-
 2 * 37089, MS, multiangle light scattering Pseudomonas aeruginosa
3.1.3.75 74600
-
 multiangle light scattering Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.75 Pseudomonas aeruginosa Q9HTR2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.75 from solubilized inclusion bodies, ion-exchange chromatography (Q-Sepharose), dialysis, gel filtration Pseudomonas aeruginosa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.3.75 dialysis, buffer with 5 mM Mg2+ Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.75 p-nitrophenyl phosphate + H2O
-
 Pseudomonas aeruginosa p-nitrophenol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.75 homodimer 2 * 37089, MS, multiangle light scattering Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.1.3.75 phosphorylcholine phosphatase
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.75 additional information
-
 apparent Tm value: 65.8°C (5 mM Mg2+) Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.75 83
-
 p-nitrophenyl phosphate
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.75 5.2
-
 maximum of kcat-value and minimum for KM-value Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.75 24
-
 p-nitrophenyl phosphate C94A mutant protein Pseudomonas aeruginosa
3.1.3.75 29
-
 p-nitrophenyl phosphate wild-type protein Pseudomonas aeruginosa
3.1.3.75 44
-
 p-nitrophenyl phosphate C87A mutant protein Pseudomonas aeruginosa